Exposure of RAW 264.7 cells to LPS for four hours dramatically increased the secretion of IL-6 to 613.2 35.1 pg/ml and TNF- to 1556.3 23.3 pg/ml
(2005) demonstrated that biotinylated GHK peptide incorporated into a collagenous matrix enhanced dermal wound healing in rat models, confirming that the GHK sequence retains biological activity when formulated into biomaterial scaffolds
Weeks 3-4: More noticeable effects typically emerge
Journal of Nutrition, 10, 112- 120
Primary antibodies against SLC7A11 (Cell Signaling Technology, 12691, 1:500 dilution), iNOS (BD Biosciences, 610329, 1:500 dilution), 15LO1 (Abnova, H00000246-D01P, 1:1000 dilution), GPX4 (Abcam, 125066, 1:1000 dilution), POSTN (Santa Cruz Biotechnology, 67233, 1:500), and CCL26 (R&D Systems, AF653, 1:500 dilution) were used