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It is based on assumption that 50% 8% of the NADP + content in frozen blood accounts for NADPH that oxidized during the freeze-thaw cycle

Rambaldi A, Saconato HH, Christensen E, Thorlund K, Wetterslev J, Gluud C

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Aitken RJ, Curry BJ (2011) Redox regulation of human sperm function: from the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line