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Comparing the physiological effects of a ketogenic diet to those of long-term fasting [27:45]

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A 10 l qPCR reaction was amplified in duplicate wells for each sample 4.5 l of cDNA mixture (100 ng cDNA template + rest nuclease-free water) with 5 l iTaq Universal SYBR Green Supermix, and 0.5 l forward and reverse primer mixtures (10 pm)