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Then they were transfer to a polyvinylidene fluoride (PVDF) membrane ( Transmission electron microscopy (TEM) The frozen section, measuring approximately 1 mm 3 , was initially fixed in 2.5% glutaraldehyde (SUPPLIES, United States, 02607-BA) within a phosphate buffer (0.1 M, pH 7.0)

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Stimulation of glucagon release by addition of anti-somatostatin serum to islet of Langerhans in vitro