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Secondary mass spectra were obtained using data-dependent acquisition (DDA, Top3) mode with multi-stage fragmentation energy settings

NAPQI-GST Formation by RLM Incubation RLM (1 mg/mL protein) and APAP (100 M) were incubated (37C and 500 rpm) in the presence of NADP + (500 M), and a NADPH-regenerating system: glucose 6-phosphate (10 mM), glucose-6-phosphate dehydrogenase (2 U/mL) and magnesium chloride (5 mM)

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p53 exerts a tumor-suppressive function when exposed to DNA damage and various endogenous/exogenous stresses (Pietsch et al., 2008)