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ThermoFisher, 11320033) with 2 mM L-glutamine, 10 mM HEPES (Sigma-Aldrich, H3375-25g), 50 U ml 1 penicillinstreptomycin, 1 B27 (ThermoFisher, 12587-010), 1 N-2 (ThermoFisher, 17502-048), 50 ng ml 1 EGF (Peprotech, AF-100-15) and 100 ng ml 1 Noggin (Peprotech, 250-38)

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Experiments conducted using a polyacrylamide-based microchannel device that allows independent control of substrate stiffness and channel dimensions revealed that optimal migration of triple-negative MDA-MB-231 and SUM159 human breast cancer cells occurs at intermediate stiffness [90]

Beyond glucose lowering, incretin-based therapies are increasingly positioned within the broader cardiometabolic framework, with emerging data supporting cardiovascular and renal protection, as well as effects on endothelial function, atherosclerosis, heart failure phenotypes, and chronic kidney disease progression

The same procedure was replicated after a 24-h interval