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The system was set to a flow rate of 0.060 mL/min and composed of 5 mM ammonium formate in a gradient of water/acetonitrile concentrations (all mobile phase solvents were Optima LC/MS Grade, and purchased from Fisher Scientific, Fair Lawn, NJ)

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If you are stable at 5 mg and accidentally inject 10 mg because you grabbed a 20 mg/mL vial instead of your usual 10 mg/mL, you have doubled your dose without the gradual titration your body expects

[73] Superoxide dismutase is known to reverse fibrosis, possibly through de-differentiation of myofibroblasts back to fibroblasts