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Briefly, cells were disrupted (Sonics Vibra Cell, Sonics & Materials Inc., Newtown, CT, USA) in an ice-cold mixture of methanol (1.66 ml) and chloroform (0.83 ml) for 3 minutes
Store at 15-25C, in a closed container, in a dry place
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This loop perpetuates inflammation and tissue damage, driving COPD progression
Les taux de GSH ont augment de 40 % dans le sang total et de 100 % dans les cellules mononucles du sang priphrique en deux semaines
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