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Impact of ascorbate-glutathione cycle components on the effectiveness of embryogenesis induction in isolated microspore cultures of barley and triticale
Similarly, in SAGA complex, TAFs assemble into distinct specific heterodimeric pairs through Histone fold domain interactions: TAF6/TAF9, Ada1/TAF12 and Spt7/TAF10 that form the architectural scaffold of the complex (5, 7-10)
Siddharth 2012
Immunoblot analysis For detection of Gclc (Santa Cruz Biotechnology sc-390811), Pdha-1 (Abcam ab168379), DRP1 (Abcam ab184247), eIF4E (Abcam ab33766), p-eIF4E (Abcam ab76256), 4E-BP1 (Cell signaling 9452), p-4E-BP1 (Cell signaling 2855), DRP1 (Abcam ab184247), mTOR (Cell Signaling 2972), p-mTOR (Ser2448) (Cell Signaling 2971), p70 S6 (Cell Signaling 9202), p-p70 S6 (Thr421/Ser424) (Cell Signaling 9204), 15 10 6 cells were lysed with lysis buffer (CST 9803S) and protein/phosphatases inhibitors as per manufacturers protocol and blotted as described 44
