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To determine how the circadian clock affects mitochondrial metabolism in adipose tissue, we isolated mitochondria from the gonadal white adipose tissue (gWAT) of male mice entrained to a normal or inverted light cycle at different times of the night and day (zeitgeber time (ZT))

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Enzyme-linked immunosorbent assay (ELISA) Culture media of 3T3-L1 adipocytes were collected for ELISA to measure the level of secreted adiponectin

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