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The percentage of living cells was determined by measuring the absorbance of the wells at 570 nm in a multiplate spectrophotometer (Spectramax, Molecular Devices, California, USA)

5 mmol l 1 sodium dithionite was added to produce the reducing conditions specified in the aforementioned methods, and the effects of CORM3 or RuCl 3 at 10 mol l 1 were examined

Cost Per Month on the Low-Dose Protocol On the community-reported low-dose protocol, so little mass is used that cost is governed by the ~28-day stability window a fresh vial is started roughly monthly regardless of dose, so smaller vials or staged reconstitution minimize waste rather than mass driving spend

Constipation is one of the most common complaints patients bring up, affecting up to 24% of users in clinical trials

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