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The salt-buffered gels were then added to 100 L reaction volumes containing a final concentration of 1X Superscript IV RT buffer, 5 mM DTT, 5 mM dNTPs, 5% ( v/v ) RNAase Inhibitor and 5% ( v/v ) Superscript IV reverse transcription enzyme and subjected to single cycle of reverse transcription (i.e

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doi:10.1177/0333102418821661 Hamel E

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