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Two additional washes, one in a 2 lysis buffer (1 M NaCl, 2 mM EDTA, 20 mM Tris pH 7.5 supplemented with 0.04% ( v/v ) SDS and 0.05% ( v/v ) Tween-20) and another in 2 lysis buffer supplemented with 20% ( v/v ) RNAse inhibitor, were performed to buffer match the gels and the spacer

These individuals benefit most from injectable B12 supplementation, whether through their tirzepatide formulation or separate B12 injections