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Brains were sliced on a sliding microtome (Leica), and serial 30 M coronal sections were collected in antifreeze and stored at 20 C until further processing

An individual might experience a confusing mix of slow digestion and irritation, leading to significant stomach pain or dehydration from diarrhea

Beyond transient soreness, uncommon but noteworthy adverse events include nodules, infection, or allergic reactions

Keep in mind that these downstream effects are based on deduction, so theyre largely hypothetical

The temporal pattern of cfos activation in hypothalamic, cortical, and brainstem nuclei in response to fasting and refeeding in male mice