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Not touching the vial directly to avoid freezing, which can denature peptides

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During the ISR, with global protein translation suppressed, untranslated mRNA transcripts and aggregation-prone RNA binding proteins such as Ras GTPase-activating protein-binding protein 1/2 (G3BP1/2) and T-cell-restricted intracellular antigen-1 related protein (TIAR) are packaged into non-membrane bound foci known as stress granules (SGs) [8]