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GHK-Cu upregulated the Nrf2/Keap1 pathway Nrf2 is attached to Keap1 in the cytoplasm under normal circumstances, while in the condition of stress, the stimuli of oxidative stress activate the separation of Nrf2 and Keap1 and translocate Nrf2 to the nucleus and switch on transcriptional activities (Itoh et al., 1997)