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In experiments using melatonin, either melatonin (10mg/kg, Sigma) or an equivalent volume of DMSO (Sigma) was intraperitoneally injected each day for three days at 17:00, immediately after intranasal challenges had been performed

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As a rough guide, this equates to approximately 500 kcal for many women and 600 kcal for many men, though these figures are illustrative rather than fixed targets