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The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)

Furthermore, analysis of methylation and expression profiles across various ethnic groups underscores the widespread occurrence of these epigenetic alterations

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Doi:10.1016/j.jhep.2024.06.003

As you continue to lose weight and maintain lower blood sugar levels, your metabolism can become more and more effective