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To characterize the Tn5-knockout reference libraries in the various ECOR strains, genomic DNA was extracted from 5 to 10 l of the cell-scrape and prepared for sequencing using the NEBNext Ultra II FS DNA Library Prep Kit (NEB) with the following modifications

Indeed, GGT1 has been studied in the context of kidney cancer, with pro-tumour and antitumour effects being attributed 54,55,56

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Eur Rev Med Pharmacol Sci 2013

Three behavioral assessments were performed in the autism behaviors and severity domain: 1) Autism Diagnostic Observation Schedule (ADOS), 2) Childhood Autism Rating Scale (CARS) and 3) the Autism Diagnostic Interview- Revised (ADI-R)