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The membranes were blocked using Bullet Blocking One for Western Blotting (Nacalai Tesque), incubated overnight at 4C with the primary antibody, and then incubated with an HRP-conjugated secondary antibody

10.1017/S0007114510004319 7

They showed Mn overexposure reduced dopamine concentrations in the striatum, accompanied by impaired motor performance (Ivleva et al., 2022

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If youre feeling unusually tired, your hair is thinning, or you experience any of the other symptoms mentioned above, it may make sense to get your vitamin levels tested