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sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml
The SURMOUNT-5 trial data you may have seen comparing tirzepatide and semaglutide head-to-head showed tirzepatide outperforming semaglutide at approximately 20.2% vs
Retrouves dans 20 40 % des cas au cours des pancratites aigus ncrosantes, les complications infectieuses sont responsables de 50 80 % des dcs
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