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This supernatant was then subjected to a second centrifugation at 9000 g for 30 min at 4 C, yielding a crude mitochondrial fraction

When taken at therapeutic doses, approximately 90% of paracetamol is metabolised through conjugation pathways that produce harmless compounds excreted in urine

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Under oxidative stress, excessive ROS emissions lead to mitochondrial damage, and loss or defects in mitochondrial energy production can enhance metabolic processes in the tricarboxylic acid (TCA) cycle, ETC, exacerbating ROS release and reducing antioxidant enzymes [81,82,83]