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MIN-6 cells were transfected with constructs encoding the human FLAG-tagged GLP-1R in the hygromycin-resistant vector pcDNA3.1 with Lipofectamine 2000 (Thermo Fisher Scientific, Massachusetts, USA) 3,8,43

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Adults who are exploring weight management options, says Rahmanian, especially people who may have been interested in GLP-1 treatments, but were discouraged by access issues or stigma. Barkley himself is stressing age

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