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Splenocytes from -MYC mice and wild-type littermates were isolated as previously described [53] and stained with APC-conjugated rat anti-mouse CD45R/B220 (ref 561880) and PE-conjugated rat anti-mouse CD43 (ref 561857) antibodies, both from BD Biosciences, before flow cytometry analysis with a FACS Canto II flow cytometer (BD Biosciences) employing Kaluza software (Beckman Coulter)
Am J Physiol Endocrinol Metab 2007
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Billy Tollett: That's right
The principle of using the minimum effective intervention applies, particularly given the lack of clinical trial data supporting combination therapy and SmPC cautions against coadministration with other weight-loss medicines
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