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doi: 10.1007/s00213-010-2094-2 47 LampreaM.CardenasF.SilveiraR.MoratoS.WalshT

Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle

CRISPRCas9-mediated gene knockout Knockout of ERN1 (gene name of IRE1) in human cell lines was performed using single guide-RNAs (sgRNAs) based on the pSpCas9(BB)-2A-Puro (PX459) (Addgene Plasmid #48139) vector developed previously 88

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