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After sample application, the column was eluted with a linear gradient from 0 to 40% ACN in 0.1% trifluoroacetic acid at a flow rate of 25 mL/min for 30 min, and wavelength 215 nm of the effluent was monitored

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Similarly, Hypoxia-induced high mobility group proteins 1 (HMGB1) can activate the c-Jun N-terminal kinase (JNK), which, in turn, stimulates the HIF-1/VEGF signaling axis, facilitating angiogenesis (44)

The hormone promotes gluconeogenesis (glucose production) in the liver and reduces glucose uptake in peripheral tissues, creating an insulin-resistant state

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