The primers used were 5-caccatcacctcctggaaga-3 and 5-gggtgcagaatctcgagttg-3 for Pck1 , 5-gtggcagtggtcggagact-3 and 5-acgggcgttgtccaaac-3 for G6pc , 5-tacagagtgctggccaagag-3 and 5-ttcaaggatgcactggagag-3 for Cyp7a1 , 5-tagccctctttcctccactcata-3 and 5-gaagcgatcgaacctaaattcct-3 for Cyp7b1 , 5-gcctcacctatgggatcttca-3v and 5v-tcaaagcctgacgcagatg-3v for Cyp27a1 , 5-ggctggcttcctgagcttatt-3 and 5-acttcctgaacagctcatcgg-3 for Cyp8b1 , 5-acctgtctaacctcttcacc-3 and 5-caatgctgaggttcatgtcc-3 for Slc10a1 , 5-ggaagattgttggcccgatt-3 and 5-agtgggagttatggtcaggt-3v for Slco1a1 , 5-gcgtcgtgattagcgatga-3 and 5-atggcctcccatctcctt-3 for Hprt
Injection dosages are usually around 1,000 mcg while oral supplement dosages can range from 50 to 5,000 mcg
B12 shots dont always resolve the underlying problem of deficiency, so theyre not the be-all and end-all when it comes to treating symptoms
This guide covers the full dosing protocol, reconstitution, injection technique, and cycling strategy based on clinical protocols used at our Doral, Miami practice
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