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Individual cardiac vagal neurons in the nucleus ambiguus were identified by the presence of the fluorescent tracer using a Zeiss Axioskop upright microscope (Carl Zeiss, Inc., Thornwood, NY, USA) using a 40 water immersion objective

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However, imaging cannot definitively distinguish between benign and malignant peripheral nerve tumors

Sally Ward (Cancer Sciences Unit, Centre for Cancer Immunology, University of Southampton, Southampton, United Kingdom), and further incubated for 72 h at 2324 C and 160 RPM

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