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To understand the underlying molecular mechanisms, the expression levels of 3-AR mRNA in both white and brown adipose tissue were assessed using reverse transcription followed by PCR and Southern blotting

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This interaction site is less defined but comprises the glutathione moiety of Grx(SSG), parts of helix 3, and a conserved lysine/arginine residue that also contributes to the scaffold site 14,15

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As such GGT plays an integral role in GSH homeostasis and has been traditionally regarded as a component of the cellular defense system against oxidative stress (Pompella et al., 2007)