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After 7 days, cells were washed with PBS, fixed with 4% paraformaldehyde for 15 min, and stained with alcian blue 8GX solution for 30 min at RT to detect intracellular glycosaminoglycan (GAG)

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The clinical literature reports typical adult dosing ranges and FDA-approved labeling exists where applicable, yet translating those into a personal dose is a clinical task, not a chart lookup