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Notice that in this example, the attacking nucleophile is an alcohol rather than an amine (thats why the enzyme is called an O-methyltransferase)

These benefits address some of the key challenges in managing type 2 diabetes, making these medications a game-changer in the field

In eight of ten colonies tested, the introduced URA3 gene could no longer be detected (Figure S3B), and none of the negative clones grew on minimal agar without added uracil

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