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hGPX4-1-F: 5-GATCCGTGAGGCAAGACCGAAGTAAACTCGAGTTTACTTCGGTCTTGCCTCACTTTTTG-3 hGPX4-1-R: 5-AATTCAAAAAGTGAGGCAAGACCGAAGTAAACTCGAGTTTACTTCGGTCTTGCCTCACG-3 hGPX4-2-F: 5-GATCCGGGAGTAACGAAGAGATCAAACTCGAGTTTGATCTCTTCGTTACTCCCTTTTTG-3 hGPX4-2-R: 5-AATTCAAAAAGGGAGTAACGAAGAGATCAAACTCGAGTTTGATCTCTTCGTTACTCCCG-3 hGPX4-3-F: 5-GATCCACGTCAAATTCGATATGTTCACTCGAGTGAACATATCGAATTTGACGTTTTTTG-3 hGPX4-3-R: 5-AATTCAAAAAACGTCAAATTCGATATGTTCACTCGAGTGAACATATCGAATTTGACGTG-3 Determination of intracellular Fe2+ levels For Liperfluo staining (Dojindo, Kumamoto, Japan), Raji cells were seeded in a 48-well plate
A summary of possible NAD + metabolite fluxes in the human body based on the mice studies summarized below is proposed in Fig
Glutathione participation in the prevention of cardiovascular diseases
Servicebio Technology) and counterstained with hematoxylin for 5 min at 25C
Furthermore, mitochondrial NADPH production is dependent on at least 5 mitochondrial enzymes including nicotinamide nucleotide transhydrogenase (NNT), isocitrate dehydrogenase 2 (IDH2), malic enzyme 3 (ME3), mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase (ALDH1L2) and methylenetetrahydrofolate dehydrogenase 1 like (MTHFD1L) (4)
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