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Vial fell or was shaken accidentally If a reconstituted vial was shaken vigorously (dropped, thrown in a bag, held by a toddler), some peptide degradation may have occurred

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The samples were fixed with 4% PFA at room temperature for 30 min, permeabilized with 0.1% Triton-100 at room temperature for 15 min, and blocked with 5% BSA solution at 37 C for 1 h

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