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In fact, in cells undergoing lethal oxidative stress, cellular protein-SSG levels show a positive relationship with GSSG levels 39

The intracellular glutamate concentration is nearly 10-fold higher than the Km value, but intracellular cysteine concentrations approximate the apparent Km value [10] , indicating that cysteine may be a limiting substrate for GCL

Mix gently

Cell supernatants were collected by centrifugation at 12,400 g for 20 min and analyzed with an ATP ELISA Kit or HMGB1 ELISA Kit following the manufacturers protocol

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