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Obesity 32 , 22552263 (2024)

Samples were then loaded onto custom-made C18 StageTips (AttractSPE Disk Bio C18-100.47.20, Affinisep) for desalting

ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10L, 5-50L, 20-200L, 200-1000L) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well

Over time, if this happens consistently, treatment may be less effective than expected

This is significantly higher than the proportion of dissatisfied family members with pneumonia, only 3 %