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Oligonucleotides were mixed in an equimolar ratio in 100 mM Tris, pH 7.5, 40 mM MgCl 2, and annealed in a thermal cycler, with denaturation at 94 C, followed by a slow temperature gradient from 90 C to 4 C

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doi: 10.2353/ajpath.2007.060441

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