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Specifically, in the morphine-induced constipation model mice, we demonstrate that morphine inhibits PVN Glu neuronal activity through MOR, thereby reducing DMV Ach neuronal activity, which leads to decreased small intestinal motility and a reduced transit rate, ultimately manifesting in constipation
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After 48 hours, cells were selected for hygromycin B resistance (Roche Diagnostics, Barcelona, Spain), and clones appeared after 1015 days
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