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Early pharmacokinetic studies suggested that when standard, unprotected glutathione was ingested, it was rapidly cleaved into its constituent amino acids (glutamate, cysteine, and glycine) by digestive enzymes like $\gamma$-glutamyltransferase and harsh stomach acids

Briefly, 0.5mL of 50% ( w / v ) trichloro acetic acid was used to precipitate the protein in colon homogenate

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