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The purification of GST fusions from mammalian cells using GSH-coupled magnetic beads usually requires the removal of the competitively binding endogenous GSH ( In brief, we develop a gene transfection-positive cell sorting system utilizing GPI-anchored GST as the affinity tag that enables positive cell isolation using GSH magnetic beads

The second line of defense in bacteria is the active expulsion of strong antibiotics through the cell wall via the cell membrane

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