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Description

The bacteria were collected by centrifugation, and the obtained pellet resuspended in Ni-NTA buffer (50 mM Tris pH 8.0, 250 mM NaCl, 40 mM imidazole, 4 mM MgCl 2 , 10 M GDP, 1 mM Tris(2-chlorehyl)phosphate (TCEP), and 5 % glycerol)

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In addition, their symptoms often overlap, so we have reason to believe that they may be connected through the gut-thyroid axis

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