The generation of intracellular ROS was visualized by capturing fluorescence images using a fluorescence microscope (Nanoscope systems, Daejeon-si, South Korea)
Following 30 min of incubation at 4 C, extracts were cleared by centrifugation (17,000 g , 20 min, 4 C) and then supernatants were analysed by liquid chromatography and mass spectrometry (LCMS)
In addition, SAMe is used in the production of the mood elevating neurotransmitter serotonin as a methyl donor
10.1002/fsn3.4312 Contributor Information Muzzamal Hussain, Email: [email protected]
Custom GSH Adduct SynthesisControlled Thiol and Terminal FunctionalizationPurified Conjugates for DMPK, Toxicology, and Probe Research Develop custom glutathione conjugates with a workflow built for research teams working in drug metabolism, reactive metabolite assessment, analytical reference standard preparation, and probe development