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8.1.4 Research limitations and considerations While the evidence is compelling, several limitations must be acknowledged, including the reliance on observational studies for human data, potential confounding factors in epidemiological investigations, and the extrapolation challenges from animal models to human populations

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For experiments involving caspase-3/7 staining, CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON)

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Batch-by-Batch Protection TruShield requires testing for every production batch or lot, ensuring consistent verification rather than one-time product approval