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Tissue was then cut into small pieces and shaken in dissociation buffer (PBS: 2% FBS, 1.0 mM DTT, 5 mM EDTA, 15 mM HEPES) for 30 min at 37 C to release epithelial cell fraction

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Prolactin elevation similarly confounds reproductive, immune, and metabolic research endpoints, as prolactin receptors are expressed across a wide tissue distribution including liver, adipose, and immune cells