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For the IFN-I bioassay, 5 10 4 3T3 cells containing an interferon-sensitive response element (ISRE) fused to luciferase 51 were plated per well into 96-well white-bottom plates (Greneir 655083) in DMEM containing 10% FBS, 100 U/ml penicillin and 100 g/ml streptomycin

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doi: 10.1161/CIRCRESAHA.121.318063