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5-Amino-1MQ was developed as a research tool to selectively block NNMT in vitro and in vivo, primarily by the Watowich group at the University of Texas at Austin

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Some studies have reported improvements in gross clinical assessment and microscopic cartilage features when AOD9604 is combined with existing interventions

At the enzymatic level, AOD 9604 has been shown to promote triglyceride hydrolysis while inhibiting lipogenesis through modulation of acetyl-CoA carboxylase activity