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The reduction of ferric to ferrous ions at low pH by non-enzymatic antioxidants was measured using a plate reader (Tecan Infinite 200, Tecan Group Ltd., Mnnedorf, Switzerland) by determining the changes in absorbance at 593 nm and the formation of blue colored ferrous-tripyridyltriazine complexes after incubation at 37C for 15 min

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It is unclear how much weight is lost with other forms of semaglutide that are used off-label for weight loss, but we would expect lower doses found in Ozempic (commonly prescribed as 1/2 1 mg) to provide less weight loss than Wegovy