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As DM progresses, patients are more likely to develop various vascular complications and experience many pathological changes, such as endothelial dysfunction, AS, and microcirculatory disorders that interact with each other, consequently leading to the development of DPD

Some lyophilization processes create denser powder requiring 5-10 minutes hydration before complete dissolution

Results become noisy, inconclusive, and hard to reproduce

PMID 25024695