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Second, the atherogenic properties of Lp(a) and LDL-C itself result in increased stiffness of the arterial walls, raising the cardiac afterload and thus heightening the risk of heart failure [103]

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Cell culture studies HEK293T cells (CRL-3216, ATCC) were cultured in DMEM (11995073, Life Technologies) supplemented with 10% heat-inactivated FBS (10500064, Life Technologies), 100 IU ml 1 penicillin and 100 g ml 1 streptomycin solution (Pen/Strep, P4333, Sigma Aldrich)