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First, while antibiotic suppression is used to invoke a role for gut microbiota in the generation of the metabolite(s) monitored, antibiotics can also have direct effects on the host, altering normal metabolism (57, 58)

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5355 In the established model of insulin secretion, islet beta-cells glucokinase firstly senses that the serum concentration of glucose is 5mM or greater, which triggers glycolysis leading to ATP production, glucose oxidation and ultimately insulin secretion