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Additionally, the secondary bile acids 3-oxoLCA and isoLCA function as TGR5 agonists that suppress the differentiation of Th17 cells in vitro through two mechanisms: (1) directly inhibiting the expression of RORt, and (2) promoting the polarization of M2 macrophages, which indirectly inhibits the development of Th17 cells (119)

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After 20 min, 10 mL of the TFET mixture is added to the peptide hydrazide solution to cause in-situ thiolysis of the peptidyl azide generated from Preparation 33